TPP1 ELISA Kit (Tripeptidyl Peptidase I)

Details for Product TPP1 ELISA Kit No. ABIN4884857, Supplier: Log in to see
Antigen
  • cln2
  • fa01b09
  • im:7149243
  • wu:fa01b09
  • CLN2
  • LPIC
  • TPP-1
  • TPP-I
  • Cln2
  • tripeptidyl peptidase I
  • tripeptidyl-peptidase 1
  • tripeptidyl peptidase 1
  • tpp1
  • NAEGRDRAFT_78259
  • MCYG_00184
  • MGYG_05881
  • MGYG_00757
  • TPP1
  • Tpp1
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
9
5
2
2
2
2
2
1
Method Type
Sandwich ELISA
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Custom Human Tripeptidyl-peptidase I (TPP1) ELISA Kit.
Sample Type Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA antibody pair recognizes human Tripeptidyl-peptidase I. Other species not yet determined.
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmids, Primers & others TPP1 products on genomics-online (e.g. as negative or positive controls)
Antigen Tripeptidyl Peptidase I (TPP1)
Alternative Name Tripeptidyl-Peptidase I (TPP1 ELISA Kit Abstract)
Gene ID 1200
UniProt O14773
Pathways Cell Division Cycle, ER-Nucleus Signaling
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
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