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ICAM1 ELISA Kit

ICAM1 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4885240
  • Target See all ICAM1 ELISA Kits
    ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
    Reactivity
    • 9
    • 8
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human ICAM-1 (CD54) SpeedELISA Kit.
    Brand
    SpeedELISA
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Characteristics
    • Compressed workflow with only one wash step and three incubation steps
    • 3 hours total processing time
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Streptavidin-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Capture Antibody
    • HRP Conjugated Detection Antibody
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Sample Volume
    100 μL
    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 70 μL of the Antibody Cocktail to each well.
    3. Incubate 10 minutes at RT.
    4. Add 50 μL of sample or standard to each well.
    5. Incubate 2 h at RT.
    6. Wash Wells
    7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    8. Incubate 30 min at RT.
    9. Add 50 μL of Stop Solution to each well.
    10. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
    Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    Expiry Date
    6 months
  • Target See all ICAM1 ELISA Kits
    ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
    Alternative Name
    ICAM-1 (ICAM1 Products)
    Synonyms
    BB2 ELISA Kit, CD54 ELISA Kit, P3.58 ELISA Kit, Icam-1 ELISA Kit, Ly-47 ELISA Kit, MALA-2 ELISA Kit, ICAM ELISA Kit, ICAM-1 ELISA Kit, ICAM1 ELISA Kit, intercellular adhesion molecule 1 ELISA Kit, intercellular adhesion molecule-1 ELISA Kit, ICAM1 ELISA Kit, Icam1 ELISA Kit, ICAM-1 ELISA Kit
    Target Type
    Viral Protein
    Gene ID
    3383
    UniProt
    P05362
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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