IGF1R ELISA Kit

Catalog No. ABIN4885550

Product Details IGF1R ELISA Kit

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Mouse IGF-1R ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Mouse IGF-1R .

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: IGF-1 R (IGF1R Products)

Synonyms: CMT2N ELISA Kit, CD221 ELISA Kit, IGFIR ELISA Kit, IGFR ELISA Kit, JTK13 ELISA Kit, IGFIRC ELISA Kit, IGFR1 ELISA Kit, IGF-1R ELISA Kit, IGF1R ELISA Kit, DKFZp469I0618 ELISA Kit, igf-1r ELISA Kit, xIGF-1R ELISA Kit, xIGFR ELISA Kit, xigf1r ELISA Kit, A330103N21Rik ELISA Kit, D930020L01 ELISA Kit, hyft ELISA Kit, igf-ir ELISA Kit, IGF-IR ELISA Kit, IGF-IRb ELISA Kit, wu:fb03h06 ELISA Kit, IGF-IRa ELISA Kit, sb:eu1090 ELISA Kit, wu:fi20b10 ELISA Kit, alanyl-tRNA synthetase ELISA Kit, insulin like growth factor 1 receptor ELISA Kit, insulin-like growth factor 1 receptor ELISA Kit, insulin-like growth factor 1 ELISA Kit, insulin-like growth factor I receptor ELISA Kit, insulin like growth factor 1 receptor S homeolog ELISA Kit, insulin-like growth factor 1b receptor ELISA Kit, insulin-like growth factor 1a receptor ELISA Kit, AARS ELISA Kit, IGF1R ELISA Kit, Igf1r ELISA Kit, igf1r ELISA Kit, Igf1 ELISA Kit, LOC780503 ELISA Kit, LOC100136427 ELISA Kit, igf1r.S ELISA Kit, igf1rb ELISA Kit, igf1ra ELISA Kit

Gene ID: 16001

UniProt: Q60751

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy