DDR2 ELISA Kit (Discoidin Domain Receptor tyrosine Kinase 2)

Details for Product DDR2 ELISA Kit No. ABIN4886038
Antigen
  • MIG20a
  • NTRKR3
  • TKT
  • TYRO10
  • AW495251
  • Ntrkr3
  • tyro10
  • DDR2
  • Tyro10
  • ddr2
  • si:ch211-193c2.1
  • discoidin domain receptor tyrosine kinase 2
  • discoidin domain receptor family, member 2
  • discoidin domain receptor family, member 2, like
  • DDR2
  • Ddr2
  • ddr2l
Epitope
pTyr740, total
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
5
2
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Options
Purpose Human Phospho-DDR2 (Tyr740) and Total DDR2 ELISA Kit. This assay semi-quantitatively measures phophorylated DDR2 (Tyr740) and Total DDR2 in lysate samples.
Sample Type Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity This ELISA kit recognizes Human DDR2 phosphorylated at site Tyrosine-740 as well as total DDR2.
Characteristics
  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Plasmids, Primers & others Plasmids, Primers & others DDR2 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name DDR2 (DDR2 ELISA Kit Abstract)
Gene ID 4921
UniProt Q16832
Pathways RTK Signaling
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months
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