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NLRP2 ELISA Kit

NLRP2 Reactivity: Human pSer343 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN4886219
  • Target See all NLRP2 ELISA Kits
    NLRP2 (NLR Family, Pyrin Domain Containing 2 (NLRP2))
    Binding Specificity
    pSer343
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Phospho-NBS1 (S343) ELISA Kit. This assay semi-quantitatively measures phophorylated NBS1 (Ser343) in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human NBS1 phosphorylated at site Serine-343.
    Characteristics
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all NLRP2 ELISA Kits
    NLRP2 (NLR Family, Pyrin Domain Containing 2 (NLRP2))
    Alternative Name
    NBS1 (NLRP2 Products)
    Synonyms
    CLR19.9 ELISA Kit, NALP2 ELISA Kit, NBS1 ELISA Kit, PAN1 ELISA Kit, PYPAF2 ELISA Kit, NLRP2 ELISA Kit, E330007A02Rik ELISA Kit, Nalp2 ELISA Kit, NLR family pyrin domain containing 2 ELISA Kit, NACHT, LRR and PYD domains-containing protein 2 ELISA Kit, NLR family pyrin domain containing 7 ELISA Kit, NLR family, pyrin domain containing 2 ELISA Kit, NLRP2 ELISA Kit, LOC100301026 ELISA Kit, LOC100387349 ELISA Kit, NLRP7 ELISA Kit, Nlrp2 ELISA Kit
    Gene ID
    55655
    UniProt
    Q9NX02
    Pathways
    Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome
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