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Phospholipase C gamma 2 ELISA Kit

PLCG2 Reactivity: Human, Mouse pTyr753 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN4886258
  • Target See all Phospholipase C gamma 2 (PLCG2) ELISA Kits
    Phospholipase C gamma 2 (PLCG2)
    Binding Specificity
    pTyr753, total
    Reactivity
    • 6
    • 3
    • 2
    Human, Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human and Mouse Phospho-PLCG2 (Tyr753) and Total PLCG2 ELISA Kit. This assay semi-quantitatively measures phophorylated PLCG2 (Tyr753) and Total PLCG2 in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    This ELISA kit recognizes Human and Mouse PLCG2 phosphorylated at site Tyrosine-753 as well as total PLCG2.
    Characteristics
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product PLCG2 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all Phospholipase C gamma 2 (PLCG2) ELISA Kits
    Phospholipase C gamma 2 (PLCG2)
    Alternative Name
    PLCG2 (PLCG2 Products)
    Synonyms
    PLCgamma2 ELISA Kit, Plcg-2 ELISA Kit, APLAID ELISA Kit, FCAS3 ELISA Kit, phospholipase C, gamma 2 ELISA Kit, phospholipase C gamma 2 ELISA Kit, phospholipase C, gamma 2 (phosphatidylinositol-specific) ELISA Kit, Plcg2 ELISA Kit, PLCG2 ELISA Kit
    Gene ID
    5336
    UniProt
    P16885, Q8CIH5
    Pathways
    RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling
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