IL12 ELISA Kit

Catalog No. ABIN4986931

Mouse IL12 ELISA Kit, Colorimetric assay for quantification of Mouse IL12.

Quick Overview for IL12 ELISA Kit (ABIN4986931)

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.02-1.0 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details IL12 ELISA Kit

Minimum Detection Limit: 0.02 ng/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Mouse IL-12p70 Ligand

Sensitivity: 7 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12p70

Background: Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of a human B-lymphoblastoid cell line (1-3). Biologically active mouse IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. While the p40 and p35 subunits by themselves do not have IL-12 activity, the p40 homodimer has been shown to bind the IL-12 receptor and is an IL-12 antagonist (4, 5). Mature mouse p35 subunit is composed of 193 amino acid (aa) residues and contains seven cysteines plus one potential N-linked glycosylation site (6). Mature mouse p40 subunit has 313 aa, with 13 cysteines and five potential N-linked glycosylation sites (6). Mouse p35 and p40 subunits show 63 % and 72 % aa identity, respectively, to the human p35 and p40 subunits (3, 6). Although mouse IL-12 is active on both human and mouse cells, human IL-12 is only active on human cells. IL-12 has been shown to have multiple effects on T lymphocytes and natural killer (NK) cells. Some of these effects include the induction of IFN-γ and TNF production by T and NK cells, the enhancement of cytotoxic activity of T and NK cells and the stimulation of T and NK cell proliferation. IL-12 has also been shown to be a central mediator of the cell-mediated immune response by promoting Th1 development (7-11). Cell surface staining for IL-12 on a human monocytic and a mouse macrophage cell line has been reported, suggesting that membrane-associated IL-12 may exist (12). Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. Although a human B cell line has been shown to produce IL-12(2), fresh B cells are apparently not producers of IL-12.

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome