PD-L1 ELISA Kit

Catalog No. ABIN4993798

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PD-L1.

Quick Overview for PD-L1 ELISA Kit (ABIN4993798)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 5.58 pg/mL - 15000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details PD-L1 ELISA Kit

Minimum Detection Limit: 5.58 pg/mL

Purpose: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human B7-H1.

Analytical Method: Quantitative

Specificity: B7H1

Components:

• Plate
• Standard
• Diluent

Material not included: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: protect your eyes

Storage: 4 °C

Storage Comment: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: B7H1

Background: B7 homolog 1 (B7-H1), also known as programmed death-ligand 1 (PD-L1) and cluster of differentiation 274 (CD274), is a 40 kDa type I transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. The binding of B7-H1 to its receptor transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes.

Gene ID: 60533

NCBI Accession: NM_021893

UniProt: Q9EP73

Pathways: Cancer Immune Checkpoints