EGF ELISA Kit

Catalog No. ABIN5026920

Human EGF ELISA Kit, Colorimetric assay for quantification of Human EGF.

Quick Overview for EGF ELISA Kit (ABIN5026920)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.9-250 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details EGF ELISA Kit

Minimum Detection Limit: 3.9 pg/mL

Purpose: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensitivity: 2.34 pg/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human EGF concentrations in serum, plasma. Detection Range: 3.9 - 250 pg/mL Sensitivity: <2.34 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human EGF antibody was pre-coated into 96-well plates. Biotin conjugated anti-human EGF detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EGF amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human EGF can be calculated using the standard curve.  

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Storage Comment: Please refer to the Manual

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF (Epidermal Growth Factor)

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation