MMP2 ELISA Kit
Quick Overview for MMP2 ELISA Kit (ABIN5026923)
Target
See all MMP2 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
-
-
Minimum Detection Limit
- 0.781 ng/mL
-
Purpose
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
-
Analytical Method
- Quantitative
-
Sensitivity
- 0.469 ng/mL
-
Characteristics
- This immunoassay kit allows for the in vitro quantitative determination of Human MMP-2 concentrations in serum, plasma. Detection Range: 0.781 - 50 ng/mL Sensitivity: < 0.469 ng/mL
-
Components
- plate, standard, diluent, antibodies
-
Material not included
- pipettes, tubes, reader, incubator
-
-
Matrix Metalloproteinase 2 (MMP2) ELISA Kit
MMP2 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Horse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Pig Colorimetric Sandwich ELISA 47 ng/mL - 3000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Human Colorimetric Sandwich ELISA 0.5 ng/mL - 32 ng/mL Plasma, Serum, Tissue Homogenate
-
-
Sample Volume
- 100 μL
-
Assay Time
- 3 - 4 h
-
Plate
- Pre-coated
-
Protocol
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human MMP-2 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human MMP-2 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MMP-2 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human MMP-2 can be calculated using the standard curve.
-
Restrictions
- For Research Use only
-
-
-
Precaution of Use
- protect your eyes
-
Storage
- 4 °C
-
Storage Comment
- Please refer to the Manual
-
-
- MMP2 (Matrix Metalloproteinase 2 (MMP2))
-
Alternative Name
- MMP-2 (Matrix Metalloproteinase 2)
-
Pathways
- Activation of Innate immune Response
Target See all MMP2 ELISA Kits
-
