IL-5 ELISA Kit
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- Target See all IL-5 (IL5) ELISA Kits
- IL-5 (IL5) (Interleukin 5 (IL5))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 15.6-1000 pg/mL
- Minimum Detection Limit
- 15.6 pg/mL
- Application
- ELISA
- Purpose
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-5 concentrations in serum, plasma and other biological fluids.
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Sensitivity
- < 9.4 pg/mL
- Components
- plate, standard, diluent, antibodies
- Material not included
- pipettes, tubes, reader, incubator
- Featured
- Discover our best selling IL5 ELISA Kit
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- Discover our top product IL5 ELISA Kit
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- Sample Volume
- 100 μL
- Assay Time
- 3 - 4 h
- Plate
- Pre-coated
- Protocol
- This assay employs an antibody specific for Anti-humanIL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Restrictions
- For Research Use only
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- Precaution of Use
- protect your eyes
- Storage
- 4 °C
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- Target See all IL-5 (IL5) ELISA Kits
- IL-5 (IL5) (Interleukin 5 (IL5))
- Alternative Name
- IL-5 (Interleukin 5) (IL5 Products)
- Synonyms
- EDF ELISA Kit, IL-5 ELISA Kit, TRF ELISA Kit, Il-5 ELISA Kit, IL5 ELISA Kit, interleukin 5 ELISA Kit, IL5 ELISA Kit, Il5 ELISA Kit
- Pathways
- JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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