IL-9 ELISA Kit

Catalog No. ABIN5026958

The Human IL-9 ELISA Kit (ABIN5026958) is a Colorimetric ELISA Kit designed to quantify Human IL-9.

Quick Overview for IL-9 ELISA Kit (ABIN5026958)

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL-9 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: This assay employs an antibody specific for Anti-human IL-9 coated on a 96-well plate.

Analytical Method: Quantitative

Sensitivity: 9.4 pg/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human IL-9 concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol:  This assay employs an antibody specific for Anti-human IL-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Storage Comment: Please refer to the Manual

Target Details for IL-9

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Alternative Name: IL-9 (Interleukin-9)

Pathways: JAK-STAT Signaling