IL-13 ELISA Kit

Catalog No. ABIN5026963

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-13.

Quick Overview for IL-13 ELISA Kit (ABIN5026963)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL-13 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensitivity: 9.4 pg/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human IL-13 concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-13 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-13 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-13 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human IL-13 can be calculated using the standard curve. 

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Storage Comment: Please refer to the Manual

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: IL-13 (Interleukin 13)

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport