IL-8 ELISA Kit

Catalog No. ABIN5068074

Human IL-8 ELISA Kit Colorimetric assay for quantification of Human IL-8.

Quick Overview for IL-8 ELISA Kit (ABIN5068074)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Culture Lysate, Plasma, Serum

Product Details IL-8 ELISA Kit

Purpose: This IL-8 ELISA is a 2.5 hour solid phase immunoassay readily applicable to measure IL-8 levels in serum, plasma, cell culture supernatant and other biological fluids in the range of 50-1600pg/mL. It showed no crossreactivity with human IL-1b, EGF, MCAF, RANTES, and SAA. This IL-8 ELISA is expected to be effectively used for further investigations into the relationship between IL-8 and various diseases.

Analytical Method: Quantitative

Sensitivity: > 10 pg/mL

Characteristics: Interleukin 8, Human (IL-8) ELISA Kit

Components:

• Microtiter Plate, 1x96 wells
• Anti-human IL-8 (HRP), 1x15ml
• * IL-8 Standard, Recombinant, 2x1vial
• Calibrator Diluent I, 1x22ml
• Calibrator Diluent II, 1x22ml
• Wash Buffer (20X), 1x60ml
• Substrate A, 1x11ml
• Substrate B, 1x11ml
• Stop Solution, 1x14ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• This IL-8 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal specific for IL-8. Standards or samples are then added to the appropriate microtiter plate wells and incubated. IL-8, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IL-8 and other components of sample. In order to quantitate the amount of IL-8 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for IL-8 is added to each well to "sandwich" the IL-8 immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies. A TMB (3,3&
• 39,5,5&
• 39, tetramethyl- benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-8 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution. The color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm. In order to measure the concentration of IL-8 in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) vs. IL-8 concentration (pg/ml). The concentration of IL-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: Interleukin 8

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy