IL-4 ELISA Kit

Catalog No. ABIN5068123

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-4.

Quick Overview for IL-4 ELISA Kit (ABIN5068123)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Purified Protein

Product Details IL-4 ELISA Kit

Analytical Method: Quantitative

Sensitivity: 6.6 pg/mL

Characteristics: Interleukin 4, Human (IL-4) ELISA Kit

Components:

• Microtiter Plate 1x96wells
• Mab (Biotin) 1x7ml
• Avidin (HRP) 1x14ml
• * Standard 2x1vial
• Calibrator Diluent I 1x22ml For serum/plasma testing
• Calibrator Diluent II 1x22ml For culture supernatant
• Wash Buffer, 20X 1x60ml
• Substrate A 1x10ml
• Substrate B 1x10ml
• Stop Solution 1x14ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• This IL-4 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a polyclonal antibody specific to IL-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-4 and incubated. IL-4 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-4 and other components of the sample. In order to quantitate the amount of IL-4 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each have a high affinity binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3&
• 39,5,5&
• 39, tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-4, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: Interleukin 4

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation