IL-13 ELISA Kit

Catalog No. ABIN5068130

The Human IL-13 ELISA Kit (ABIN5068130) is a Colorimetric ELISA Kit designed to quantify Human IL-13.

Quick Overview for IL-13 ELISA Kit (ABIN5068130)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details IL-13 ELISA Kit

Analytical Method: Quantitative

Cross-Reactivity (Details): Calculated cross reactivity: Hu

Sensitivity: > 32 pg/mL

Characteristics: Interleukin 13 (IL-13) ELISA Kit (Human)

Components:

• Microtiter Plate, 1x96 wells
• Pab (HRP), 1x21ml
• Standard*, 1x20ng
• Assay Diluent: 1x11ml
• (Cell Culture Supernatant), 1x21ml
• Calibrator Diluent (Serum/Plasma), 1x21ml
• Wash Buffer (25X), 1x21ml
• Color Reagent A (2), 1x12.5ml
• Color Reagent B (TMB), 1x12.5ml
• Stop Solution, 1x6ml

Application Details

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-13 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IL-13 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: Interleukin 13 (IL-13)

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport