MFGE8 ELISA Kit
Quick Overview for MFGE8 ELISA Kit (ABIN5068339)
Target
See all MFGE8 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Analytical Method
- Quantitative
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Sensitivity
- 13.4 pg/mL
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Characteristics
- MFG-E8, Human, ELISA Kit (BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, SED1)
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Components
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- Mfg-e8 Microplate, 1x96wells
- Mfg-e8 Standard, lyophilized, 1x40ng
- Mfg-e8 Conjugate, 1x21ml
- Assay Diluent r, 1x11ml
- Calibrator Diluent r, 1x21ml
- Wash Buffer Concentrate, 25X concentrated, 1x21ml
- Color Reagent A, 1x12ml
- Color Reagent B, 1x12ml
- Stop Solution, 1x6ml
- Plate Sealers, 4x adhesive strips
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Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA Kit
MFGE8 Reactivity: Human Colorimetric Sandwich ELISA 781.25 pg/mL - 50000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Urine
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reactivity: Human AA 24-387 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reactivity: Mouse AA 23-463 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reactivity: Human Colorimetric Sandwich ELISA 0.1-25 ng/mL Cell Culture Supernatant, Plasma, Serum
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Plate
- Pre-coated
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Protocol
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Principle:
- This employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human MFG-E8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any human MFG-E8 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for human MFG-E8 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human MFG-E8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Restrictions
- For Research Use only
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Storage
- 4 °C,-20 °C
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Storage Comment
- 4°C/-20°C
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- MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
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Alternative Name
- MFG-E8
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Background
- MFG-E8, originally identified as a product of lactating mammary glands, is also secreted by activated macrophages and immature dendritic cells. MFG-E8 functions as an opsonin by binding phosphatidylserine on apoptotic cells and enhancing their phagocytosis.
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Pathways
- SARS-CoV-2 Protein Interactome
Target See all MFGE8 ELISA Kits
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