EGF ELISA Kit

Catalog No. ABIN5068374

Human EGF ELISA Kit, Colorimetric assay for quantification of Human EGF.

Quick Overview for EGF ELISA Kit (ABIN5068374)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Urine, Plasma, Cell Culture Supernatant, Serum

Product Details EGF ELISA Kit

Sensitivity: > 20 pg/mL

Characteristics: Epidermal Growth Factor, Human (EGF), ELISA Kit

Components:

• Microtiter Plate 1x96wells
• . EGF (HRP) 1x15ml
• * EGF Standard 2x4ng
• Calibrator Diluent I (serum/plasma) 1x22ml
• Calibrator Diluent II (cell culture supernatant/urine) 1x22ml
• Wash Buffer (20X) 1x60ml
• Substrate A 1x11ml
• . Substrate B 1x11ml
• Stop Solution, O4, 1x14ml

Application Details

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: Epidermal Growth Factor

Background: Epidermal growth factor (EGF), a polypeptide mitogen, was first observed in 1959 by Cohen and Levi-Montalcini while studying Nerve Growth Factor (NGF) in snake venom extracts.1 It was subsequently isolated and purified from mouse submandibular glands. When injected into new-born mice this new factor caused precocious eyelid opening and incisor eruption.2,3 EGF was further purified, based on its ability to induce the proliferation of basal skin cells.4 Also, a potent inhibitor of gastric acid secretion was identified and isolated from the urine of a pregnant women, and named human ß-urogastrone. It was shown that this protein was very similar to purified human EGF.5,6

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation