Leptin ELISA Kit
Quick Overview for Leptin ELISA Kit (ABIN5068384)
Target
See all Leptin (LEP) ELISA KitsReactivity
Detection Method
Application
Sample Type
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Sensitivity
- 0.5 ng/mL
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Characteristics
- Leptin, Serum, Human, ELISA Kit (Ob, Obese Protein, Obesity Factor)
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Components
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- Microwell Strip Plate, 1x96wells
- Standard, 0ng/ml, 1x10ml
- Standard, 1ng/ml, 1x500ul
- Standard, 5ng/ml, 1x500ul
- Standard, 10ng/ml, 1x500ul
- Standard, 20ng/ml, 1x500ul
- Standard, 50ng/ml, 1x500ul
- Standard, 100ng/ml, 1x500ul
- Low Control, 1x500ul
- 0: High Control, 1x500ul
- 1: AB (Biotin), 1x10ml
- 2: Streptavidin (HRP) 50X, 1x400ul
- 3: Assay Buffer, 1x20ml
- 4: Wash Buffer, 10X, 1x50ml
- 5: TMB, 1x16ml
- 6: Stop Solution, 1x6ml
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Leptin (LEP) ELISA Kit
LEP Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Salmon Colorimetric Competition ELISA 62.5 pg/mL - 1000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP Reactivity: Rabbit Colorimetric Competition ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Rabbit Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Chicken Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP Reactivity: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Pig Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C
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- Leptin (LEP)
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Alternative Name
- Leptin
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Background
- Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Recently, Ob genes (mouse and human) have been cloned. Obese gene encodes an adipocyte-tissue derived secreted Ob protein/Leptin (167aa, ~16kD) that controls body weight homeostasis. Exogenous administration of recombinant Ob protein can reduce food intake and body weight. However, Ob protein had no effect in db/db mice suggesting a defect in leptin signaling mechanism. Leptin exert its effects by interacting with leptin receptors (obese receptors). The Ob-R has been shown to be a product of db gene that has long been thought to encode the receptor for a weight-controlling hormone. The Ob-R has at least 6 alternatively spliced forms with modifications at the amino and C-terminus.
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Pathways
- JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
Target See all Leptin (LEP) ELISA Kits
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