Insulin ELISA Kit

Catalog No. ABIN5520034

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse Insulin. There is 1 publication available.

Quick Overview for Insulin ELISA Kit (ABIN5520034)

Target: Insulin (INS)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details Insulin ELISA Kit

Minimum Detection Limit: 78.125 pg/mL

Purpose: For quantitative detection of INS in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of INS. No significant cross-reactivity or interference between INS and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between INS and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 46.875 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

References for Insulin ELISA Kit (ABIN5520034)

Xiao, Wen, Ning, Zhai, Liao, Lin, Mu, Bian: "Cyclocarya paliurus tea leaves enhances pancreatic β cell preservation through inhibition of apoptosis." in: Scientific reports, Vol. 7, Issue 1, pp. 9155, (2017) (PubMed).

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: INS

Background: INS(Insulin)

UniProt: P01325

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation