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SP1 ELISA Kit

The Rat SP1 ELISA Kit (ABIN5521622) is a Colorimetric ELISA Kit designed to quantify Rat SP1.
Catalog No. ABIN5521622
$643.65
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 13 to 17 Business Days

Quick Overview for SP1 ELISA Kit (ABIN5521622)

Target

See all SP1 ELISA Kits
SP1 (Sp1 Transcription Factor (SP1))

Reactivity

  • 21
  • 12
  • 10
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

78.125 pg/mL - 5000 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
  • Minimum Detection Limit

    78.125 pg/mL

    Purpose

    Rat Sp1 (Transcription factor Sp1) ELISA Kit

    Analytical Method

    Quantitative

    Specificity

    Specifically binds with Sp1 , no obvious cross reaction with other analogues.

    Sensitivity

    46.875 pg/mL

    Components

    • ELISA Microplate(Dismountable)
    • Lyophilized Standard
    • Biotin-labeled Antibody
    • HRP-Streptavidin Conjugate(SABC
    • 100X)
    • TMB Substrate
    • Sample Dilution Buffer
    • Stop Solution
    • Plate Sealer
    • Product Description

    Material not included

    • Microplate reader (wavelength: 450nm)
    • 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
    • Automated plate washer or multi-channel pipette/5 mL pipettor
    • Precision single (0.5-10μL
    • 5-50μL
    • 20-200μL
    • 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
    • Sterile tubes and Eppendorf tubes with disposable tips
    • Absorbent paper and loading slot
    • Deionized or distilled water
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    Sandwich ELISA, Double Antibody

    Assay Time

    4 h

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Handling Advice

    Avoid direct light exposure to antibody solutions

    Storage

    4 °C

    Storage Comment

    Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

    Expiry Date

    12 months
  • Target See all SP1 ELISA Kits

    SP1 (Sp1 Transcription Factor (SP1))

    Alternative Name

    Sp1

    Background

    Transcription factor Sp1, SP1, TSFP1

    UniProt

    Q01714

    Pathways

    Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction
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