HES1 ELISA Kit

Catalog No. ABIN5522088

Human HES1 ELISA Kit Colorimetric assay for quantification of Human HES1.

Quick Overview for HES1 ELISA Kit (ABIN5522088)

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details HES1 ELISA Kit

Minimum Detection Limit: 78.125 pg/mL

Purpose: For quantitative detection of HES1 in cell lysates, tissue homogenates and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of HES1. No significant cross-reactivity or interference between HES1 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between HES1 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 46.875 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for HES1

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Alternative Name: HES1

Background: HES1/HL/HRY/BHLHB39/bHLHb39hairy homolog(Drosophila)/Class B basic helix-loop-helix protein 39/Hairy and enhancer of split 1/hairy and enhancer of split 1,(Drosophila)/Hairy homolog/Hairy-like protein/Hes1/HES-1/hHL/HL/HRYHHL/transcription factor HES-1

UniProt: Q14469

Pathways: DNA Damage Repair