SIRT1 ELISA Kit

Catalog No. ABIN5522221

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat SIRT1.

Quick Overview for SIRT1 ELISA Kit (ABIN5522221)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details SIRT1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Purpose: For quantitative detection in serum, plasma, tissue homogenates and other biological fluids.

Analytical Method: Quantitative

Specificity: SIRT1/SIR2alpha/SIR2L1/SIR2-like protein 1/EC 3.5.1/S. cerevisiae, homolog) 1/sirtuin 1

Sensitivity: 0.094 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4 °C for 6 months

Expiry Date: 6 months

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background: SIRT1/SIR2alpha/SIR2L1/SIR2-like protein 1/EC 3.5.1/S. cerevisiae, homolog) 1/sirtuin 1

UniProt: A0A0G2JZ79

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling