NLRC4 ELISA Kit

Catalog No. ABIN5522369

The Human NLRC4 ELISA Kit (ABIN5522369) is a Colorimetric ELISA Kit designed to quantify Human NLRC4.

Quick Overview for NLRC4 ELISA Kit (ABIN5522369)

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details NLRC4 ELISA Kit

Minimum Detection Limit: 78.125 pg/mL

Purpose: For quantitative detection of NLRC4 in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of NLRC4. No significant cross-reactivity or interference between NLRC4 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between NLRC4 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 46.875 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for NLRC4

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Alternative Name: NLRC4

Background: NLRC4/NLR family CARD domain-containing protein 4/Ice protease-activating factor/Ipaf/CARD, LRR, and NACHT-containing protein/Clan protein/Caspase recruitment domain-containing protein 12

UniProt: Q9NPP4

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome