Reticulon 4 ELISA Kit

Catalog No. ABIN5522679

Human Reticulon 4 ELISA Kit Colorimetric assay for quantification of Human Reticulon 4 and has been mentioned in 1 publication.

Quick Overview for Reticulon 4 ELISA Kit (ABIN5522679)

Target: Reticulon 4 (RTN4)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details Reticulon 4 ELISA Kit

Minimum Detection Limit: 78.125 pg/mL

Purpose: For quantitative detection of RTN4 in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of RTN4. No significant cross-reactivity or interference between RTN4 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between RTN4 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 46.875 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

References for Reticulon 4 ELISA Kit (ABIN5522679)

Koper, Kamińska, Milewska, Sawicki, Mariak, Kemona, Matowicka-Karna: "The isoform A of reticulon-4 (Nogo-A) in cerebrospinal fluid of primary brain tumor patients: influencing factors." in: Oncotarget, Vol. 9, Issue 38, pp. 25048-25056, (2018) (PubMed).

Target Details for Reticulon 4

Target: Reticulon 4 (RTN4)

Alternative Name: RTN4

Background: RTN4/Reticulon-5/RTN-x/Neuroendocrine-specific protein C homolog/Neuroendocrine-specific protein(NSP)/Foocen/Neurite outgrowth inhibitor(Nogo protein)/

UniProt: Q9NQC3

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome