HSD3B1 ELISA Kit
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- Target See all HSD3B1 ELISA Kits
- HSD3B1 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Detection Range
- 0.313 ng/mL - 20 ng/mL
- Minimum Detection Limit
- 0.313 ng/mL
- Application
- ELISA
- Purpose
- For quantitative detection of HSD3B1 in serum, plasma, tissue homogenates.
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of HSD3B1. No significant cross- reactivity or interference between HSD3B1 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between HSD3B1 and all the analogues, therefore, cross reaction may still exist.
- Sensitivity
- 0.188 ng/mL
- Components
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- ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Competitive ELISA, Coated with Antigen
- Sample Volume
- 50 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
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- For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
- For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
- Expiry Date
- 6 months
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- Target See all HSD3B1 ELISA Kits
- HSD3B1 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1))
- Alternative Name
- HSD3B1 (HSD3B1 Products)
- Synonyms
- 3BETAHSD ELISA Kit, HSD3B ELISA Kit, HSDB3 ELISA Kit, HSDB3A ELISA Kit, I ELISA Kit, SDR11E1 ELISA Kit, HSD3B1 ELISA Kit, MGC131206 ELISA Kit, D3Ertd383e ELISA Kit, 3B-HSD ELISA Kit, 3BHSD ELISA Kit, si:rp71-68n21.10 ELISA Kit, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 ELISA Kit, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 L homeolog ELISA Kit, HSD3B1 ELISA Kit, LOC469446 ELISA Kit, LOC713091 ELISA Kit, hsd3b1.L ELISA Kit, LOC100451655 ELISA Kit, Hsd3b1 ELISA Kit, hsd3b1 ELISA Kit
- Background
- HSD3B1/3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I(3-beta-HSD I)/Trophoblast antigen FDO161G/3BH/HSDB3A
- UniProt
- P14060
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis
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