Ki-67 ELISA Kit

Catalog No. ABIN5524359

Human Ki-67 ELISA Kit Colorimetric assay for quantification of Human Ki-67.

Quick Overview for Ki-67 ELISA Kit (ABIN5524359)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.313 ng/mL - 20 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details Ki-67 ELISA Kit

Minimum Detection Limit: 0.313 ng/mL

Purpose: For quantitative detection of MKI67 in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of MKI67. No significant cross-reactivity or interference between MKI67 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between MKI67 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.188 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: MKI67

Background: MKI67/Antigen KI-67/Ki-67/KIA/TSG126/antigen identified by monoclonal Ki-67/KIA/proliferation-related Ki-67 antigen

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process