Caspase 9 ELISA Kit

Catalog No. ABIN5524393

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Caspase 9.

Quick Overview for Caspase 9 ELISA Kit (ABIN5524393)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.313 ng/mL - 20 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details Caspase 9 ELISA Kit

Minimum Detection Limit: 0.313 ng/mL

Purpose: For quantitative detection of CASP9 in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of CASP9. No significant cross-reactivity or interference between CASP9 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between CASP9 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.188 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: CASP9

Background: CASP9/Caspase 9/Apoptotic protease Mch 6/p35/p10/Cas9/APAF-3/APAF3/apoptotic protease activating factor 3/Apoptotic protease Mch-6/Apoptotic protease-activating factor 3/CASP-9/apoptosis-related cysteine peptidase/caspase-9/ICE-LAP6/CASPASE-9c/ICE-like ap

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity