HNF4A ELISA Kit

Catalog No. ABIN5524621

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse HNF4A.

Quick Overview for HNF4A ELISA Kit (ABIN5524621)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 39.063 pg/mL - 2500 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details HNF4A ELISA Kit

Minimum Detection Limit: 39.063 pg/mL

Purpose: For quantitative detection of HNF4a in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of HNF4a. No significant cross-reactivity or interference between HNF4a and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between HNF4a and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 23.438 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4a

Background: HNF4a/HNF4A/MODY1/NR2A1/TCF14/FLJ39654/hepatic nuclear factor 4 alpha/hepatocyte nuclear factor 4, alpha/hepatocyte nuclear factor 4-alpha/HNF4a7/HNF-4-alpha/HNF4alpha10/11/12/HNF4HNF4a8/MODY/MODY1/NR2A1HNF4a9/NR2A21/Nuclear receptor subfamily 2 group A m

UniProt: P49698

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process