Topoisomerase II alpha ELISA Kit

Catalog No. ABIN5525034

Product Details Topoisomerase II alpha ELISA Kit

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.313 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.313 ng/mL

Application: ELISA

Purpose: For quantitative detection of TOP2A in serum, plasma, tissue homogenates.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of TOP2A. No significant cross-reactivity or interference between TOP2A and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between TOP2A and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.188 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A (TOP2A Products)

Synonyms: TOP2 ELISA Kit, TP2A ELISA Kit, TOP2A ELISA Kit, Top-2 ELISA Kit, TOP-2 ELISA Kit, F12B17.110 ELISA Kit, F12B17_110 ELISA Kit, thimet metalloendopeptidase 2 ELISA Kit, fc08c07 ELISA Kit, wu:fc08c07 ELISA Kit, DNA topoisomerase II alpha ELISA Kit, DNA topoisomerase II, alpha isozyme ELISA Kit, topoisomerase (DNA) II alpha ELISA Kit, Zincin-like metalloproteases family protein ELISA Kit, TOP2A ELISA Kit, Bm1_08705 ELISA Kit, Top2a ELISA Kit, AT5G10540 ELISA Kit, top2a ELISA Kit

Background: TOP2A/TOP2/TP2A/DNA topoisomerase II, alpha isozyme/DNA topoisomerase(ATP-hydrolyzing)/DNA topoisomerase 2-alpha/TOP2DNA gyrase/topoisomerase(DNA) II alpha(170kD)/topoisomerase(DNA) II alpha 170  kDa

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases