PRKAA1 ELISA Kit (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit)

Details for Product PRKAA1 ELISA Kit No. ABIN5526721, Supplier: Log in to see
Antigen
  • AMPK
  • AMPKa1
  • cb116
  • im:7154392
  • sb:cb130
  • wu:fa94c10
  • AMPK1
  • AMPKalpha1
  • AI194361
  • AI450832
  • AL024255
  • C130083N04Rik
  • ampk
  • ampka1
  • PRKAA1
  • protein kinase AMP-activated catalytic subunit alpha 1
  • protein kinase, AMP-activated, alpha 1 catalytic subunit
  • protein kinase, AMP-activated, alpha 1 catalytic subunit L homeolog
  • PRKAA1
  • prkaa1
  • Prkaa1
  • prkaa1.L
Epitope
pSer487
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
13
6
6
1
Method Type
Sandwich ELISA
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Human, Mouse and Rat Phospho-AMPKa1 (Ser487) ELISA Kit. This assay semi-quantitatively measures AMPKa1 phosphorylated at Serine-487 in cell lysate samples.
Sample Type Cell Culture Lysate
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity This ELISA kit recognizes Human, Mouse and Rat AMPKa1 phosphorylated at site Serine-487.
Characteristics
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Plasmids, Primers & others Plasmids, Primers & others PRKAA1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name AMPKa1 (PRKAA1 ELISA Kit Abstract)
Gene ID 5562
UniProt Q13131, Q5EG47, P54645
Pathways AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
Application Notes Optimal working dilution should be determined by the investigator.
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months
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