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Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D) ELISA Kit ELISA Kit

INPP5D Reactivity: Human pTyr1020 Colorimetric Cell ELISA Adherent Cell Culture
Catalog No. ABIN5526727
$734.80
Plus shipping costs $45.00
96 tests
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Delivery in 21 to 31 Business Days
  • Target
    Binding Specificity
    pTyr1020, total
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Cell ELISA
    Application
    ELISA
    Purpose
    Cell-Based ELISA Kit. This assay semi-quantitatively measures SHIP1 phosphorylated at Tyrosine-1020 as well as total SHIP1 in adherent cell lines.
    Sample Type
    Adherent Cell Culture
    Analytical Method
    Semi-Quantitative
    Specificity
    This ELISA kit recognizes Human SHIP1 phosphorylated at site Tyrosine-1020 as well as total SHIP1.
    Characteristics
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    Components
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target
    Alternative Name
    SHIP1 (INPP5D ELISA Kit Abstract)
    Gene ID
    3635
    UniProt
    Q92835
    Pathways
    TCR Signaling
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