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SOX2 ELISA Kit

SOX2 Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Catalog No. ABIN5526732
  • Target See all SOX2 ELISA Kits
    SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))
    Reactivity
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    DNA-Binding ELISA
    Application
    ELISA
    Purpose
    Human SOX2 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical SOX2 binding sequences to semi-quantitatively detect active SOX2 in lysates or nuclear extracts. Only available in North America.
    Sample Type
    Cell Lysate, Nuclear Extract
    Analytical Method
    Semi-Quantitative
    Specificity
    The olionucleotide/antibody pair provided in this kit recognizes human SOX2 in whole lysates and nuclear extracts.
    Characteristics
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    Components
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product SOX2 ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    Expiry Date
    6 months
  • Target See all SOX2 ELISA Kits
    SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))
    Alternative Name
    SOX2 (SOX2 Products)
    Synonyms
    ANOP3 ELISA Kit, MCOPS3 ELISA Kit, RGD1565646 ELISA Kit, Sox-2 ELISA Kit, lcc ELISA Kit, ysb ELISA Kit, cb236 ELISA Kit, wu:fb83g04 ELISA Kit, wu:fc14d07 ELISA Kit, zgc:65860 ELISA Kit, zgc:77389 ELISA Kit, SRY-box2 ELISA Kit, Xsox-2 ELISA Kit, anop3 ELISA Kit, mcops3 ELISA Kit, sox-2 ELISA Kit, SRY-box 2 ELISA Kit, SRY box 2 ELISA Kit, SRY (sex determining region Y)-box 2 ELISA Kit, SRY-box 2 S homeolog ELISA Kit, SOX2 ELISA Kit, Sox2 ELISA Kit, sox2 ELISA Kit, sox2.S ELISA Kit
    Gene ID
    6657
    UniProt
    P48431
    Pathways
    Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis
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