LIF ELISA Kit

Catalog No. ABIN5526766

The Human LIF ELISA Kit (ABIN5526766) is a Colorimetric ELISA Kit designed to quantify Human LIF.

Quick Overview for LIF ELISA Kit (ABIN5526766)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.64 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details LIF ELISA Kit

Minimum Detection Limit: 0.64 pg/mL

Purpose: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human LIF.

Analytical Method: Quantitative

Specificity: LIF

Components:

• Plate
• Standard
• Diluent

Material not included: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: protect your eyes

Storage: 4 °C

Storage Comment: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Target Details for LIF

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background: Leukemia inhibitory factor (LIF) is an interleukin 6 family cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. Cells known to express LIF include activated T-cells, monocytes, and astrocytes, osteoblasts, keratinocytes, mast cells, and fibroblasts. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. During pregnancy LIF is involved in decidualization of the maternal endometrium and implantation of the blastocyst to the endometrium. Women with decreased production of LIF and other cytokines are fertile and able to become pregnant, but there is an increased risk for unexplained, recurrent miscarriages. It has been suggested that recombinant human LIF might help to improve the implantation rate in women with unexplained infertility. In addition, LIF is typically added to stem cell culture medium to reduce spontaneous differentiation.

Gene ID: 3976

NCBI Accession: NM_001257135

UniProt: P15018

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding