ADAM10 ELISA Kit

Catalog No. ABIN5651301

Product Details ADAM10 ELISA Kit

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 10 (ADAM10). No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 10 (ADAM10) and analogues was observed.

Sensitivity: 28 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 10 (ADAM10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 10 (ADAM10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ADAM10

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Alternative Name: A Disintegrin And Metalloprotease 10 (ADAM10 Products)

Synonyms: ADAM10 ELISA Kit, Adam10 ELISA Kit, adam10 ELISA Kit, wu:fc03d12 ELISA Kit, ad10 ELISA Kit, cd156c ELISA Kit, kuz ELISA Kit, kuzbanian ELISA Kit, madm ELISA Kit, xadam10 ELISA Kit, ADAM-10 ELISA Kit, ADAM 10 ELISA Kit, AD10 ELISA Kit, CD156c ELISA Kit, HsT18717 ELISA Kit, MADM ELISA Kit, 1700031C13Rik ELISA Kit, LOC100219653 ELISA Kit, zgc:64203 ELISA Kit, ADAM metallopeptidase domain 10 ELISA Kit, ADAM metallopeptidase domain 10a ELISA Kit, ADAM metallopeptidase domain 10 S homeolog ELISA Kit, a disintegrin and metallopeptidase domain 10 ELISA Kit, ADAM metallopeptidase domain 10b ELISA Kit, ADAM10 ELISA Kit, Adam10 ELISA Kit, adam10a ELISA Kit, adam10.S ELISA Kit, adam10b ELISA Kit

Background:

Gene Name: A Disintegrin And Metalloprotease 10

Gene Aliases: CD156-C, CD156c, MADM, Kuz, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease

Gene ID: 102

UniProt: O14672

Pathways: Notch Signaling, EGFR Signaling Pathway