ALDOA ELISA Kit

Catalog No. ABIN5651565

Product Details ALDOA ELISA Kit

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Aldolase A, Fructose Bisphosphate (ALDOA). No significant cross-reactivity or interference between Aldolase A, Fructose Bisphosphate (ALDOA) and analogues was observed.

Sensitivity: 6.7 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldolase A, Fructose Bisphosphate (ALDOA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldolase A, Fructose Bisphosphate (ALDOA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldolase A, Fructose Bisphosphate (ALDOA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aldolase A, Fructose Bisphosphate (ALDOA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldolase A, Fructose Bisphosphate (ALDOA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldolase A, Fructose Bisphosphate (ALDOA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ALDOA

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Alternative Name: Aldolase A, Fructose Bisphosphate (ALDOA Products)

Synonyms: ALDA ELISA Kit, GSD12 ELISA Kit, aldoa ELISA Kit, cb79 ELISA Kit, sb:cb79 ELISA Kit, wu:fa28b10 ELISA Kit, wu:fb10b11 ELISA Kit, ALDOA ELISA Kit, Aldo-1 ELISA Kit, Aldo1 ELISA Kit, RNALDOG5 ELISA Kit, hm:zeh0036 ELISA Kit, zgc:77696 ELISA Kit, aldolase, fructose-bisphosphate A ELISA Kit, aldolase a, fructose-bisphosphate, a ELISA Kit, aldolase, fructose-bisphosphate A S homeolog ELISA Kit, aldolase A, fructose-bisphosphate ELISA Kit, aldolase a, fructose-bisphosphate, b ELISA Kit, ALDOA ELISA Kit, aldoaa ELISA Kit, aldoa ELISA Kit, aldoa.S ELISA Kit, Aldoa ELISA Kit, aldoab ELISA Kit

Background:

Gene Name: Aldolase A, Fructose Bisphosphate

Gene Aliases: FBA, ALDO-A, Fructose 1,6 Bisphosphate Aldolase, Lung cancer antigen NY-LU-1, Muscle-type aldolase

Gene ID: 226

UniProt: P04075

Pathways: Ribonucleoside Biosynthetic Process