SPTAN1 ELISA Kit

Catalog No. ABIN5651650

Product Details SPTAN1 ELISA Kit

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Alpha-Fodrin (SPTAN1). No significant cross-reactivity or interference between Alpha-Fodrin (SPTAN1) and analogues was observed.

Sensitivity: 0.68 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fodrin (SPTAN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Fodrin (SPTAN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fodrin (SPTAN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Fodrin (SPTAN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Fodrin (SPTAN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Fodrin (SPTAN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SPTAN1

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Alternative Name: Alpha-Fodrin (SPTAN1 Products)

Synonyms: im:7157190 ELISA Kit, wu:fa20e05 ELISA Kit, wu:fb33g08 ELISA Kit, wu:fk32a10 ELISA Kit, zgc:112229 ELISA Kit, 2610027H02Rik ELISA Kit, Spna-2 ELISA Kit, Spna2 ELISA Kit, EIEE5 ELISA Kit, NEAS ELISA Kit, SPTA2 ELISA Kit, A2a ELISA Kit, IPF ELISA Kit, SPECA ELISA Kit, spectrin alpha, non-erythrocytic 1 ELISA Kit, spectrin, alpha, non-erythrocytic 1 ELISA Kit, SPTAN1 ELISA Kit, sptan1 ELISA Kit, Sptan1 ELISA Kit

Background:

Gene Name: Alpha-Fodrin

Gene Aliases: SPTAN1, NEAS, (ALPHA)II-SPECTRIN, Spectrin Alpha Non-Erythrocytic 1, Spectrin Alpha Chain,Brain, Spectrin, non-erythroid alpha subunit

UniProt: P16546

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization