Angiopoietin 2 CLIA Kit

Catalog No. ABIN5651738

Product Details Angiopoietin 2 CLIA Kit

Target: Angiopoietin 2 (ANGPT2)

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 6.9 pg/mL - 5000 pg/mL

Minimum Detection Limit: 6.9 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Angiopoietin 2 (ANGPT2). No significant cross-reactivity or interference between Angiopoietin 2 (ANGPT2) and analogues was observed.

Sensitivity: 2.9 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Angiopoietin 2 (ANGPT2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Angiopoietin 2 (ANGPT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Angiopoietin 2 (ANGPT2) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin 2 (ANGPT2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin 2 (ANGPT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: Angiopoietin 2 (ANGPT2 Products)

Synonyms: AGPT2 CLIA Kit, ANG2 CLIA Kit, Agpt2 CLIA Kit, Ang-2 CLIA Kit, Ang2 CLIA Kit, ang2 CLIA Kit, angpt2 CLIA Kit, ANGIOPOIETIN-2 CLIA Kit, ANG-2 CLIA Kit, ANGPT2 CLIA Kit, Angrp CLIA Kit, Rnase5b CLIA Kit, ANG3 CLIA Kit, C11orf2 CLIA Kit, C11orf3 CLIA Kit, FFR CLIA Kit, angiopoietin 2 CLIA Kit, angiopoietin 2b CLIA Kit, angiogenin, ribonuclease A family, member 2 CLIA Kit, VPS51, GARP complex subunit CLIA Kit, ANGPT2 CLIA Kit, Angpt2 CLIA Kit, angpt2b CLIA Kit, Ang2 CLIA Kit, VPS51 CLIA Kit

Background:

Gene Name: Angiopoietin 2

Gene Aliases: ANG2, AGPT2

Gene ID: 285

UniProt: O15123

Pathways: RTK Signaling