APAF1 ELISA Kit

Catalog No. ABIN5651962

Product Details APAF1 ELISA Kit

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Apoptotic Peptidase Activating Factor 1 (APAF1). No significant cross-reactivity or interference between Apoptotic Peptidase Activating Factor 1 (APAF1) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apoptotic Peptidase Activating Factor 1 (APAF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apoptotic Peptidase Activating Factor 1 (APAF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptotic Peptidase Activating Factor 1 (APAF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptotic Peptidase Activating Factor 1 (APAF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: Apoptotic Peptidase Activating Factor 1 (APAF1 Products)

Synonyms: APAF1 ELISA Kit, ARK ELISA Kit, Apaf-1 ELISA Kit, Apaf1 ELISA Kit, CG6829 ELISA Kit, D-Apaf-1 ELISA Kit, DARK ELISA Kit, Dapaf-1 ELISA Kit, Dapaf-1/HAC-1 ELISA Kit, Dark ELISA Kit, Dark/Apaf-I ELISA Kit, Dark/Dapaf-1/HAC1 ELISA Kit, Dark/Hac-1/dApaf-1 ELISA Kit, Dark/Hac-1/dApaf1 ELISA Kit, Dmel\\CG6829 ELISA Kit, Hac-1 ELISA Kit, Hac-1/Dark ELISA Kit, Hac1 ELISA Kit, T1 ELISA Kit, anon-53Fa ELISA Kit, apaf-1 ELISA Kit, apaf1 ELISA Kit, arc ELISA Kit, ark ELISA Kit, dAPAF-1 ELISA Kit, dApaf-1 ELISA Kit, dApaf-1/DARK/HAC-1 ELISA Kit, dApaf1 ELISA Kit, dArk ELISA Kit, dapaf ELISA Kit, dapaf-1 ELISA Kit, dapaf-1L ELISA Kit, dapaf-1S ELISA Kit, dark ELISA Kit, dark/dapaf-1/hac-1 ELISA Kit, dark/hac-1/dapaf-1 ELISA Kit, hac-1 ELISA Kit, hac1 ELISA Kit, l(2)SH0173 ELISA Kit, APAF-1 ELISA Kit, CED4 ELISA Kit, 6230400I06Rik ELISA Kit, Apaf1l ELISA Kit, fog ELISA Kit, mKIAA0413 ELISA Kit, zgc:136543 ELISA Kit, Death-associated APAF1-related killer ELISA Kit, apoptotic peptidase activating factor 1 L homeolog ELISA Kit, apoptotic peptidase activating factor 1 ELISA Kit, Dark ELISA Kit, apaf1.L ELISA Kit, apaf1 ELISA Kit, APAF1 ELISA Kit, Apaf1 ELISA Kit

Background:

Gene Name: Apoptotic Peptidase Activating Factor 1

Gene Aliases: CED4, Apoptotic Protease Activating Factor

Gene ID: 317

UniProt: O14727

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity