BNIP3 CLIA Kit

Catalog No. ABIN5652170

Rat BNIP3 ELISA Kit, Chemiluminescent assay for quantification of Rat BNIP3.

Quick Overview for BNIP3 CLIA Kit (ABIN5652170)

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Reactivity: Rat

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 54.9 pg/mL - 40000 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details BNIP3 CLIA Kit

Minimum Detection Limit: 54.9 pg/mL

Purpose: Rat Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) CLIA Kit for detecting BNIP3 in tissue homogenates, cell lysates

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3). No significant cross-reactivity or interference between Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3) and analogues was observed.

Sensitivity: 22.7 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Alternative Name: Bcl2/Adenovirus E1B 19kDa Interacting Protein 3

Background: Gene Aliases: NIP3

UniProt: Q9ET45

Pathways: Autophagy, Brown Fat Cell Differentiation