BCAR1 ELISA Kit

Catalog No. ABIN5652342

Product Details BCAR1 ELISA Kit

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.12 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Breast Cancer Anti-Estrogen Resistance 1 (BCAR1). No significant cross-reactivity or interference between Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) and analogues was observed.

Sensitivity: 27 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Anti-Estrogen Resistance 1 (BCAR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Breast Cancer Anti-Estrogen Resistance 1 (BCAR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast Cancer Anti-Estrogen Resistance 1 (BCAR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Alternative Name: Breast Cancer Anti-Estrogen Resistance 1 (BCAR1 Products)

Synonyms: CAS ELISA Kit, CAS1 ELISA Kit, CASS1 ELISA Kit, CRKAS ELISA Kit, P130Cas ELISA Kit, AI385681 ELISA Kit, Cas ELISA Kit, Crkas ELISA Kit, P130CAS ELISA Kit, MGC68466 ELISA Kit, cas ELISA Kit, p130cas ELISA Kit, BCAR1 ELISA Kit, fc17f11 ELISA Kit, wu:fc17f11 ELISA Kit, zgc:175192 ELISA Kit, BCAR1, Cas family scaffolding protein ELISA Kit, breast cancer anti-estrogen resistance 1 ELISA Kit, breast cancer anti-estrogen resistance 1 L homeolog ELISA Kit, breast cancer anti-estrogen resistance protein 1 ELISA Kit, BCAR1 ELISA Kit, Bcar1 ELISA Kit, bcar1.L ELISA Kit, bcar1 ELISA Kit, LOC100435586 ELISA Kit

Background:

Gene Name: Breast Cancer Anti-Estrogen Resistance 1

Gene Aliases: CAS, CRKAS, P130Cas, CASS1, Crk-Associated Substrate, Cas Scaffolding Protein Family Member 1

Gene ID: 9564

UniProt: P56945

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling