BRCA2 ELISA Kit

Catalog No. ABIN5652346

Product Details BRCA2 ELISA Kit

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Breast Cancer Susceptibility Protein 2 (BRCA2). No significant cross-reactivity or interference between Breast Cancer Susceptibility Protein 2 (BRCA2) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Susceptibility Protein 2 (BRCA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Breast Cancer Susceptibility Protein 2 (BRCA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast Cancer Susceptibility Protein 2 (BRCA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Breast Cancer Susceptibility Protein 2 (BRCA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Breast Cancer Susceptibility Protein 2 (BRCA2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Breast Cancer Susceptibility Protein 2 (BRCA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for BRCA2

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Alternative Name: Breast Cancer Susceptibility Protein 2 (BRCA2 Products)

Synonyms: BRCC2 ELISA Kit, BROVCA2 ELISA Kit, FACD ELISA Kit, FAD ELISA Kit, FAD1 ELISA Kit, FANCB ELISA Kit, FANCD ELISA Kit, FANCD1 ELISA Kit, GLM3 ELISA Kit, PNCA2 ELISA Kit, 30169 ELISA Kit, BcDNA:SD25109 ELISA Kit, CG13583 ELISA Kit, CG13584 ELISA Kit, CG30169 ELISA Kit, Dmel\\CG30169 ELISA Kit, brca2 ELISA Kit, dmbrca2 ELISA Kit, fancd1 ELISA Kit, BRCA2 ELISA Kit, brcc2 ELISA Kit, brovca2 ELISA Kit, facd ELISA Kit, fad ELISA Kit, fad1 ELISA Kit, fancb ELISA Kit, AI256696 ELISA Kit, AW045498 ELISA Kit, Fancd1 ELISA Kit, RAB163 ELISA Kit, BRCA2, DNA repair associated ELISA Kit, Breast cancer 2, early onset homolog ELISA Kit, breast cancer 2, early onset ELISA Kit, BRCA2 ELISA Kit, Brca2 ELISA Kit, brca2 ELISA Kit, LOC584780 ELISA Kit

Background:

Gene Name: Breast Cancer Susceptibility Protein 2

Gene Aliases: BRCA2, BRCC2, FACD, FAD, FAD1, FANCD, FANCD1, Breast Cancer 2,Early Onset, Breast Cancer Type 2 Susceptibility Protein, Fanconi anemia group D1 protein

Gene ID: 12190

UniProt: P97929

Pathways: DNA Damage Repair, M Phase, Maintenance of Protein Location