CASR ELISA Kit
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- Target See all CASR ELISA Kits
- CASR (Calcium-Sensing Receptor (CASR))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Calcium Sensing Receptor (CASR). No significant cross-reactivity or interference between Calcium Sensing Receptor (CASR) and analogues was observed.
- Sensitivity
- 0.057 ng/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Sensing Receptor (CASR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Sensing Receptor (CASR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Sensing Receptor (CASR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Sensing Receptor (CASR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium Sensing Receptor (CASR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium Sensing Receptor (CASR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all CASR ELISA Kits
- CASR (Calcium-Sensing Receptor (CASR))
- Alternative Name
- Calcium Sensing Receptor (CASR Products)
- Synonyms
- CAR ELISA Kit, EIG8 ELISA Kit, FHH ELISA Kit, FIH ELISA Kit, GPRC2A ELISA Kit, HHC ELISA Kit, HHC1 ELISA Kit, HYPOC1 ELISA Kit, NSHPT ELISA Kit, PCAR1 ELISA Kit, CaR ELISA Kit, Gprc2a ELISA Kit, gprc2a ELISA Kit, xV2R F-1 ELISA Kit, CASR ELISA Kit, calcium sensing receptor ELISA Kit, calcium-sensing receptor ELISA Kit, calcium-sensing receptor L homeolog ELISA Kit, CASR ELISA Kit, Casr ELISA Kit, casr.L ELISA Kit
- Background
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Gene Name: Calcium Sensing Receptor
Gene Aliases: CAR, FHH, FIH, GPRC2A, HHC, HHC1, NSHPT, PCAR1, Parathyroid cell calcium-sensing receptor 1, Hypocalciuric Hypercalcemia 1, Severe Neonatal Hyperparathyroidism
- Gene ID
- 12374
- UniProt
- Q9QY96
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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