Complement C4 ELISA Kit

Catalog No. ABIN5653231

Product Details Complement C4 ELISA Kit

Target: Complement C4 (C4) (Complement 4 (C4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 9.38 ng/mL - 600 ng/mL

Minimum Detection Limit: 9.38 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Complement Component 4 (C4). No significant cross-reactivity or interference between Complement Component 4 (C4) and analogues was observed.

Sensitivity: 4.3 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 4 (C4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 4 (C4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 4 (C4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 4 (C4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 4 (C4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 4 (C4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Complement C4

Target: Complement C4 (C4) (Complement 4 (C4))

Alternative Name: Complement Component 4 (C4 Products)

Synonyms: C4B1 ELISA Kit, C4B12 ELISA Kit, C4B2 ELISA Kit, C4B3 ELISA Kit, C4B5 ELISA Kit, C4BD ELISA Kit, C4B_2 ELISA Kit, C4F ELISA Kit, CH ELISA Kit, CO4 ELISA Kit, CPAMD3 ELISA Kit, C4 ELISA Kit, C4A2 ELISA Kit, C4A3 ELISA Kit, C4A4 ELISA Kit, C4A6 ELISA Kit, C4AD ELISA Kit, C4S ELISA Kit, CPAMD2 ELISA Kit, RG ELISA Kit, C4-1 ELISA Kit, C4b ELISA Kit, C4-2 ELISA Kit, Slp ELISA Kit, Ss ELISA Kit, complement C4B (Chido blood group) ELISA Kit, complement C4A (Rodgers blood group) ELISA Kit, complement component 4A (Rodgers blood group) ELISA Kit, complement 4 ELISA Kit, complement component 4A ELISA Kit, complement component 4B (Chido blood group) ELISA Kit, C4B ELISA Kit, C4A ELISA Kit, C4a ELISA Kit, C4 ELISA Kit, C4b ELISA Kit

Background:

Gene Name: Complement Component 4

Gene Aliases: C4BP-a, Complement Component 4 Binding Protein,Alpha

Gene ID: 720

UniProt: P0C0L4

Pathways: Complement System