Connexin 43/GJA1 ELISA Kit

Catalog No. ABIN5653305

Product Details Connexin 43/GJA1 ELISA Kit

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Connexin 43 (CX43). No significant cross-reactivity or interference between Connexin 43 (CX43) and analogues was observed.

Sensitivity: 0.118 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connexin 43 (CX43). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Connexin 43 (CX43). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Connexin 43 (CX43), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Connexin 43 (CX43) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connexin 43 (CX43) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connexin 43 (CX43) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: Connexin 43 (GJA1 Products)

Synonyms: AVSD3 ELISA Kit, CX43 ELISA Kit, DFNB38 ELISA Kit, GJAL ELISA Kit, HLHS1 ELISA Kit, HSS ELISA Kit, ODDD ELISA Kit, Cx43 ELISA Kit, AU042049 ELISA Kit, AW546267 ELISA Kit, Cnx43 ELISA Kit, Cx43alpha1 ELISA Kit, Gja-1 ELISA Kit, Npm1 ELISA Kit, connexin43 ELISA Kit, cx43a1 ELISA Kit, gja1 ELISA Kit, zfCx43.3 ELISA Kit, connexin 43 ELISA Kit, gja1-A ELISA Kit, cx43 ELISA Kit, gjal ELISA Kit, oddd ELISA Kit, dfnb38 ELISA Kit, gap junction protein alpha 1 ELISA Kit, gap junction protein, alpha 1 ELISA Kit, connexin 43 ELISA Kit, gap junction protein alpha 1 L homeolog ELISA Kit, CONNEXIN 43 protein ELISA Kit, GJA1 ELISA Kit, Gja1 ELISA Kit, cx43 ELISA Kit, gja1.L ELISA Kit, gja1 ELISA Kit, CONNEXIN 43 ELISA Kit

Background:

Gene Name: Connexin 43

Gene Aliases: ODDD, GJAL, GJA1, GJ-A1, Gap junction 43 kDa heart protein, Gap Junction Protein Alpha 1, Oculodentodigital Dysplasia, Syndactyly Type III

Gene ID: 24392

UniProt: P08050

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization