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Coxsackie Adenovirus Receptor ELISA Kit

CXADR Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5653355
  • Target See all Coxsackie Adenovirus Receptor (CXADR) ELISA Kits
    Coxsackie Adenovirus Receptor (CXADR) (Coxsackie Virus and Adenovirus Receptor (CXADR))
    Reactivity
    • 4
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Coxsackie Virus And Adenovirus Receptor (CXADR). No significant cross-reactivity or interference between Coxsackie Virus And Adenovirus Receptor (CXADR) and analogues was observed.
    Sensitivity
    0.054 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coxsackie Virus And Adenovirus Receptor (CXADR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coxsackie Virus And Adenovirus Receptor (CXADR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coxsackie Virus And Adenovirus Receptor (CXADR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coxsackie Virus And Adenovirus Receptor (CXADR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coxsackie Virus And Adenovirus Receptor (CXADR) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coxsackie Virus And Adenovirus Receptor (CXADR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all Coxsackie Adenovirus Receptor (CXADR) ELISA Kits
    Coxsackie Adenovirus Receptor (CXADR) (Coxsackie Virus and Adenovirus Receptor (CXADR))
    Alternative Name
    Coxsackie Virus And Adenovirus Receptor (CXADR Products)
    Synonyms
    CAR ELISA Kit, CAR4/6 ELISA Kit, HCAR ELISA Kit, Car1 ELISA Kit, car ELISA Kit, cb331 ELISA Kit, fb76g12 ELISA Kit, wu:fb76g12 ELISA Kit, 2610206D03Rik ELISA Kit, AU016810 ELISA Kit, AW553441 ELISA Kit, MCAR ELISA Kit, MCVADR ELISA Kit, hcar ELISA Kit, xCAR ELISA Kit, CXADR ELISA Kit, CXADR, Ig-like cell adhesion molecule ELISA Kit, coxsackie virus and adenovirus receptor ELISA Kit, CXADR, Ig-like cell adhesion molecule L homeolog ELISA Kit, CXADR ELISA Kit, Cxadr ELISA Kit, cxadr ELISA Kit, cxadr.L ELISA Kit
    Background

    Gene Name: Coxsackie Virus And Adenovirus Receptor

    Gene Aliases: CAR, HCAR, HCVADR, CVB3-binding protein, Coxsackievirus B-adenovirus receptor

    Gene ID
    1525
    UniProt
    P78310
    Pathways
    Cell-Cell Junction Organization
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