DKK1 ELISA Kit

Catalog No. ABIN5653663

Product Details DKK1 ELISA Kit

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Dickkopf Related Protein 1 (DKK1). No significant cross-reactivity or interference between Dickkopf Related Protein 1 (DKK1) and analogues was observed.

Sensitivity: 0.27 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dickkopf Related Protein 1 (DKK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dickkopf Related Protein 1 (DKK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dickkopf Related Protein 1 (DKK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dickkopf Related Protein 1 (DKK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dickkopf Related Protein 1 (DKK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dickkopf Related Protein 1 (DKK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: Dickkopf Related Protein 1 (DKK1 Products)

Synonyms: DKK-1 ELISA Kit, SK ELISA Kit, AI414495 ELISA Kit, SGY ELISA Kit, SGY-1 ELISA Kit, SGY1 ELISA Kit, Soggy ELISA Kit, mSgy ELISA Kit, dkk1 ELISA Kit, dkk ELISA Kit, dkk-1 ELISA Kit, xdkk1 ELISA Kit, Xdkk-1 ELISA Kit, Dickkopf1 ELISA Kit, dickkopf-1 ELISA Kit, mdkk-1 ELISA Kit, dkk1-A ELISA Kit, dickkopf WNT signaling pathway inhibitor 1 ELISA Kit, dickkopf like acrosomal protein 1 ELISA Kit, dickkopf-related protein 1 ELISA Kit, dickkopf-like 1 ELISA Kit, dickkopf WNT signaling pathway inhibitor 1b ELISA Kit, dickkopf protein ELISA Kit, dickkopf WNT signaling pathway inhibitor 1 S homeolog ELISA Kit, DKK1 ELISA Kit, Dkk1 ELISA Kit, DKKL1 ELISA Kit, LOC100228789 ELISA Kit, Dkkl1 ELISA Kit, dkk1b ELISA Kit, dkk1 ELISA Kit, dickkopf ELISA Kit, dkk1.S ELISA Kit

Background:

Gene Name: Dickkopf Related Protein 1

Gene Aliases: SK, Dickkopf Homolog 1(Xenopus Laevis)

Gene ID: 108348170

UniProt: D3Z9J1

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation