DBH ELISA Kit

Catalog No. ABIN5653721

Product Details DBH ELISA Kit

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12 ng/mL - 200 ng/mL

Minimum Detection Limit: 3.12 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Dopamine Beta Hydroxylase (DbH). No significant cross-reactivity or interference between Dopamine Beta Hydroxylase (DbH) and analogues was observed.

Sensitivity: 1.31 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Beta Hydroxylase (DbH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Beta Hydroxylase (DbH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Beta Hydroxylase (DbH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopamine Beta Hydroxylase (DbH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine Beta Hydroxylase (DbH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine Beta Hydroxylase (DbH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DBH

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Alternative Name: Dopamine Beta Hydroxylase (DBH Products)

Synonyms: CG1543 ELISA Kit, DBH ELISA Kit, Dbh ELISA Kit, Dmel\\CG1543 ELISA Kit, T beta h ELISA Kit, TBH ELISA Kit, TBetaH ELISA Kit, TBetah ELISA Kit, TBh ELISA Kit, TbetaH ELISA Kit, TbetaM ELISA Kit, Tbetah ELISA Kit, tbh ELISA Kit, DBM ELISA Kit, DOPBHY ELISA Kit, Tbh ELISA Kit, dopamine beta-hydroxylase (dopamine beta-monooxygenase) ELISA Kit, Tyramine beta hydroxylase ELISA Kit, MOXD1 homolog 2 ELISA Kit, dopamine beta hydroxylase ELISA Kit, dopamine beta-hydroxylase ELISA Kit, dbh ELISA Kit, Tbh ELISA Kit, LOC5574699 ELISA Kit, CpipJ_CPIJ004736 ELISA Kit, CpipJ_CPIJ004737 ELISA Kit, CpipJ_CPIJ014202 ELISA Kit, CpipJ_CPIJ016745 ELISA Kit, CpipJ_CPIJ019034 ELISA Kit, DBH ELISA Kit, Dbh ELISA Kit, LOC101110668 ELISA Kit

Background:

Gene Name: Dopamine Beta Hydroxylase

Gene Aliases: DBM, Dopamine Beta-Monooxygenase

Gene ID: 1621

UniProt: P09172

Pathways: Carbohydrate Homeostasis