Dystrophin ELISA Kit

Catalog No. ABIN5653761

Product Details Dystrophin ELISA Kit

Target: Dystrophin (DMD)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Dystrophin (DMD). No significant cross-reactivity or interference between Dystrophin (DMD) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dystrophin (DMD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dystrophin (DMD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dystrophin (DMD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dystrophin (DMD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dystrophin (DMD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dystrophin (DMD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Dystrophin

Target: Dystrophin (DMD)

Alternative Name: Dystrophin (DMD Products)

Synonyms: DMD ELISA Kit, Dmd ELISA Kit, MGC83347 ELISA Kit, DKFZp459C1629 ELISA Kit, dmd ELISA Kit, MGC79631 ELISA Kit, DKFZp468A1620 ELISA Kit, Dp71 ELISA Kit, cb664 ELISA Kit, zfDYS ELISA Kit, im:6911785 ELISA Kit, zgc:110165 ELISA Kit, BMD ELISA Kit, CMD3B ELISA Kit, DXS142 ELISA Kit, DXS164 ELISA Kit, DXS206 ELISA Kit, DXS230 ELISA Kit, DXS239 ELISA Kit, DXS268 ELISA Kit, DXS269 ELISA Kit, DXS270 ELISA Kit, DXS272 ELISA Kit, DXSmh7 ELISA Kit, DXSmh9 ELISA Kit, Dp427 ELISA Kit, dys ELISA Kit, mdx ELISA Kit, pke ELISA Kit, DNADMD1 ELISA Kit, RATDMD ELISA Kit, CG17750 ELISA Kit, CG31175 ELISA Kit, CG34157 ELISA Kit, CG7240 ELISA Kit, CG7243 ELISA Kit, CG7344 ELISA Kit, DLP ELISA Kit, DLP1 ELISA Kit, DLP186 ELISA Kit, DLP2 ELISA Kit, DLP3 ELISA Kit, DmDLP ELISA Kit, DmDYS ELISA Kit, Dmel\\CG34157 ELISA Kit, Dp117 ELISA Kit, Dp186 ELISA Kit, Dp205 ELISA Kit, GI3046716 ELISA Kit, IDLP ELISA Kit, det ELISA Kit, dmDLP ELISA Kit, dmDp186 ELISA Kit, dmDys ELISA Kit, dystrophin ELISA Kit, dystrophin, gene 1 L homeolog ELISA Kit, dystrophin, gene 1 ELISA Kit, dystrophin, muscular dystrophy ELISA Kit, Dystrophin ELISA Kit, DMD ELISA Kit, LOC708073 ELISA Kit, dmd.1.L ELISA Kit, LOC465557 ELISA Kit, dmd.1 ELISA Kit, dmd ELISA Kit, Dmd ELISA Kit, Dys ELISA Kit

Background:

Gene Name: Dystrophin

Gene Aliases: CMD3B, Muscular Dystrophy,Duchenne And Becker Types

Gene ID: 1756

UniProt: P11532

Pathways: Skeletal Muscle Fiber Development