ETFA ELISA Kit

Catalog No. ABIN5653816

Product Details ETFA ELISA Kit

Target: ETFA (Electron-Transfer-Flavoprotein, alpha Polypeptide (ETFA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). No significant cross-reactivity or interference between Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Electron Transfer Flavoprotein Alpha Polypeptide (ETFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ETFA

Target: ETFA (Electron-Transfer-Flavoprotein, alpha Polypeptide (ETFA))

Alternative Name: Electron Transfer Flavoprotein Alpha Polypeptide (ETFA Products)

Synonyms: 2010200I21Rik ELISA Kit, D9Ertd394e ELISA Kit, ETF ELISA Kit, EMA ELISA Kit, GA2 ELISA Kit, MADD ELISA Kit, cb1020 ELISA Kit, fd06h11 ELISA Kit, wu:fd06h11 ELISA Kit, ema ELISA Kit, ga2 ELISA Kit, madd ELISA Kit, electron transfer flavoprotein alpha subunit ELISA Kit, electron-transfer-flavoprotein, alpha polypeptide ELISA Kit, putative electron-transfer-flavoprotein,alpha polypeptide ELISA Kit, electron transfer flavoprotein subunit alpha, mitochondrial ELISA Kit, electron transferring flavoprotein, alpha polypeptide ELISA Kit, electron transfer flavoprotein alpha subunit L homeolog ELISA Kit, ETFA ELISA Kit, Tc00.1047053503559.109 ELISA Kit, Tc00.1047053511693.90 ELISA Kit, LMJF_28_1140 ELISA Kit, LOC100194695 ELISA Kit, etfa ELISA Kit, Etfa ELISA Kit, etfa.L ELISA Kit

Background:

Gene Name: Electron Transfer Flavoprotein Alpha Polypeptide

Gene Aliases: EMA, GA2, MADD, Glutaric Aciduria II

Gene ID: 2108

UniProt: P13804

Pathways: SARS-CoV-2 Protein Interactome